addgene plasmid (Addgene inc)
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Addgene inc
addgene plasmid
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/espcas9+(1%2E1)+plasmid/eSpCas9(1%2E1)+(Plasmid+%2371814)/bio_rxiv__64898__2026__03__24__713622-222-20-20
Average 96 stars, based on 204 article reviews
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/espcas9+(1%2E1)+plasmid/eSpCas9(1%2E1)+(Plasmid+%2371814)/bio_rxiv__64898__2026__03__24__713622-222-20-20
Average 96 stars, based on 204 article reviews
addgene plasmid - by Bioz Stars,
2026-09
96/100 stars
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Knock-Out:Article Title: SUBCELLULAR FUNCTIONS OF UBE3A ISOFORMS DRIVE SYNAPTIC DYSFUNCTION IN ANGELMAN SYNDROME Article Snippet: .. To knock out Ube3a with CRISPR, we used an engineered spCas9 with enhanced specificity (espCas9 (1.1)), gift from Feng Zhang, Article Title: DUSP12 regulates NAT10-mediated RNA acetylation to modulate DNA repair and therapeutic response in hepatocellular carcinoma Article Snippet: Doxorubicin (Sigma), actinomycin D (Invitrogen), and remodelin (MedChem Express) were prepared as 10 mM stock solutions in DMSO, and cisplatin (Sigma) was prepared as 10 mM in 0.9% NaCl. .. DUSP12 knockout was generated using sgRNAs cloned into the eSpCas9( CRISPR:Article Title: SUBCELLULAR FUNCTIONS OF UBE3A ISOFORMS DRIVE SYNAPTIC DYSFUNCTION IN ANGELMAN SYNDROME Article Snippet: .. To knock out Ube3a with CRISPR, we used an engineered spCas9 with enhanced specificity (espCas9 (1.1)), gift from Feng Zhang, Article Title: Synchronization of the segmentation clock using synthetic cell–cell signaling Article Snippet: .. For genome editing of mouse embryonic stem cells (mESCs), we used a high-fidelity CRISPR–Cas9 nuclease: eSpCas9( Article Title: CRISPR-Cas9-based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant. Article Snippet: .. The transformation process involved the cloning of sgRNAs into Article Title: CRISPR-Cas9–based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant Article Snippet: .. The transformation process involved the cloning of sgRNAs into Plasmid Preparation:Article Title: SUBCELLULAR FUNCTIONS OF UBE3A ISOFORMS DRIVE SYNAPTIC DYSFUNCTION IN ANGELMAN SYNDROME Article Snippet: .. To knock out Ube3a with CRISPR, we used an engineered spCas9 with enhanced specificity (espCas9 (1.1)), gift from Feng Zhang, Article Title: DUSP12 regulates NAT10-mediated RNA acetylation to modulate DNA repair and therapeutic response in hepatocellular carcinoma Article Snippet: Doxorubicin (Sigma), actinomycin D (Invitrogen), and remodelin (MedChem Express) were prepared as 10 mM stock solutions in DMSO, and cisplatin (Sigma) was prepared as 10 mM in 0.9% NaCl. .. DUSP12 knockout was generated using sgRNAs cloned into the eSpCas9( Article Title: NuSAP Safeguards Centriole Integrity to Mediate CEP57‐CEP152 Torus Recruitment for Proper Engagement Article Snippet: .. Two sgRNAs (Table ) targeting the first exon of NUSAP1 (Figure ) were created and cloned into the eSpCas9( Article Title: CRISPR-Cas9-based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant. Article Snippet: .. The transformation process involved the cloning of sgRNAs into Article Title: NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement. Article Snippet: .. Two sgRNAs (Table 1 ) targeting the first exon of NUSAP1 (Figure S1A ) were created and cloned into the eSpCas9( Article Title: NIPBL-mediated 3D genome folding translates enhancer priming into gene activation and safeguards lineage fidelity during embryonic transitions Article Snippet: .. Oligonucleotide pairs were annealed and cloned into the Article Title: CRISPR-Cas9–based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant Article Snippet: .. The transformation process involved the cloning of sgRNAs into Software:Article Title: SUBCELLULAR FUNCTIONS OF UBE3A ISOFORMS DRIVE SYNAPTIC DYSFUNCTION IN ANGELMAN SYNDROME Article Snippet: .. To knock out Ube3a with CRISPR, we used an engineered spCas9 with enhanced specificity (espCas9 (1.1)), gift from Feng Zhang, Generated:Article Title: DUSP12 regulates NAT10-mediated RNA acetylation to modulate DNA repair and therapeutic response in hepatocellular carcinoma Article Snippet: Doxorubicin (Sigma), actinomycin D (Invitrogen), and remodelin (MedChem Express) were prepared as 10 mM stock solutions in DMSO, and cisplatin (Sigma) was prepared as 10 mM in 0.9% NaCl. .. DUSP12 knockout was generated using sgRNAs cloned into the eSpCas9( Clone Assay:Article Title: DUSP12 regulates NAT10-mediated RNA acetylation to modulate DNA repair and therapeutic response in hepatocellular carcinoma Article Snippet: Doxorubicin (Sigma), actinomycin D (Invitrogen), and remodelin (MedChem Express) were prepared as 10 mM stock solutions in DMSO, and cisplatin (Sigma) was prepared as 10 mM in 0.9% NaCl. .. DUSP12 knockout was generated using sgRNAs cloned into the eSpCas9( Article Title: NuSAP Safeguards Centriole Integrity to Mediate CEP57‐CEP152 Torus Recruitment for Proper Engagement Article Snippet: .. Two sgRNAs (Table ) targeting the first exon of NUSAP1 (Figure ) were created and cloned into the eSpCas9( Article Title: NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement. Article Snippet: .. Two sgRNAs (Table 1 ) targeting the first exon of NUSAP1 (Figure S1A ) were created and cloned into the eSpCas9( Article Title: NIPBL-mediated 3D genome folding translates enhancer priming into gene activation and safeguards lineage fidelity during embryonic transitions Article Snippet: .. Oligonucleotide pairs were annealed and cloned into the Transformation Assay:Article Title: CRISPR-Cas9-based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant. Article Snippet: .. The transformation process involved the cloning of sgRNAs into Article Title: CRISPR-Cas9–based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant Article Snippet: .. The transformation process involved the cloning of sgRNAs into Cloning:Article Title: CRISPR-Cas9-based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant. Article Snippet: .. The transformation process involved the cloning of sgRNAs into Article Title: CRISPR-Cas9–based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant Article Snippet: .. The transformation process involved the cloning of sgRNAs into |